Department：Obstetrics
Medical School：Zhejiang University School of Medicine, China
Academic Rank：Associate Professor
Mechanism and therapy of sepsis-induced cardiovascular injury.
Dr. Chen aims to analyze long non-coding RNA (lncRNA) and mRNA expression profiles in septic mice heart, and to identify potential lncRNAs and mRNAs that are responsible for cardiac mitochondrial dysfunction during sepsis.
Mice were treated with 10 mg/kg of lipopolysaccharides to induce sepsis. LncRNAs and mRNAs expression were evaluated by using lncRNA and mRNA microarray or real-time PCR technique. LncRNA-mRNA co-expression network assay, Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed. The results showed that 1275 lncRNAs were differentially expressed in septic myocardium compared with those in the control group. A total of 2769 mRNAs were dysregulated in septic mice heart, most of which are mainly related to the process of inflammation, mitochondrial metabolism, oxidative stress, and apoptosis. Co-expression network analysis showed that 14 lncRNAs were highly correlated with 11 mitochondria-related differentially expressed mRNA. Among all lncRNAs and their cis-acting mRNAs, 41 lncRNAs-mRNA pairs (such as NONMMUG004378 and Apaf1 gene) were enriched in GO terms and KEGG pathways.
In summary, Dr. Chen gained some specific lncRNAs and their potential target mRNAs that might be involved in mitochondrial dysfunction in septic myocardium. These findings provide a panoramic view of lncRNA and might allow developing new treatment strategies for sepsis.
Shi Y, Zheng X, Zheng M, Wang L, Chen Y*, Shen Y*. Identification of mitochondrial function-associated lncRNAs in septic mice myocardium. J Cell Biochem. 2020; 1-16.
Fu CY, Chen J, Lu XY, Zheng MZ, Wang LL, Shen YL*, Chen YY*. Dimethyl fumarate attenuates lipopolysaccharide-induced mitochondrial injury by activating Nrf2 pathway in cardiomyocytes. Life Sci. 2019; 235: 116863.
Shen YL, Shi YZ, Chen GG, Wang LL, Zheng MZ, Jin HF*, Chen YY*. TNF-α induces Drp1-mediated mitochondrial fragmentation during inflammatory cardiomyocyte injury. Int J Mol Med. 2018; 41(4):2317-2327.
Investigation on the role of mitochondrial deacetylase SIRT3 in regulating sepsis-induced myocardial metabolic remodeling. Funding Source: National Natural Science Foundation of China.
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